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一般来讲，显微镜在电脑上成像是比较清晰的，并且对拍摄的图片可以进行系统的分析。但有时也会出现一些问题，那是由哪些因素影响着它的成像呢？
1.被检样品是否符合标准
2.选择物镜应使用平场以上档次的物镜
3.孔径光阑和数值孔径相符，才能得到最佳图像
4.熟练使用调焦系统
5.机械移动平台的精度和稳定性需好的等等因素
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